| Registro completo |
| Provedor de dados: |
Neotropical Ichthyology
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| País: |
Brazil
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| Título: |
Functional integrity of Colossoma macropomum (Cuvier, 1816) sperm cryopreserved with enriched extender solutions
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| Autores: |
Garcia,Raycon Roberto Freitas
Vasconcelos,Ana Carina Nogueira
Povh,Jayme Aparecido
Oberst,Eneder Rosana
Varela Jr.,Antonio Sérgio
Corcini,Carine Dahl
Streit Jr.,Danilo Pedro
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| Data: |
2015-09-01
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| Ano: |
2015
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| Palavras-chave: |
Cryopreservation
Cryoprotectant solution
Mitochondrial functionality
Motility
Spermatic DNA
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| Resumo: |
Cryoprotectant solutions are used to protect the sperm from alterations caused by the low temperature in the cryopreservation process. We evaluated the quality of Colossoma macropomum semen after freezing, using dimethyl sulfoxide (DMSO) as a cryoprotectant, combined with two extender solutions (T1 - Solution 1: Glucose 90.0 g/L, Sodium Citrate 6.0 g/L, EDTA 1.5 g/L, Sodium Bicarbonate 1.5 g/L, Potassium Chloride 0.8 g/L, Gentamycin Sulphate 0.2 g/L, and T2 - Solution 2: Glucose 90.0 g/L, ACP(r)-104 10.0 g/L). Motility rate and motility time did not differ between T1 and T2 and were lower than fresh semen. The number of normal sperm was significantly different in treatments T1 (15.1%) and T2 (21.9%), and both showed a reduction in the percentage of normal sperm compared to fresh semen (57.4%). The values found for the rates of fertilization and hatching, mitochondrial functionality and sperm DNA, did not differ between the treatments (T1 and T2). Regarding membrane integrity, there was a higher percentage of spermatozoa with intact membranes in T1 (53.4%) than T2 (43.7%). The extender solutions, combined with 10% DMSO, maintained the sperm DNA intact in almost all the C. macropomum sperm cells, however there was a loss in their functionality.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300599
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| Editor: |
Sociedade Brasileira de Ictiologia
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| Relação: |
10.1590/1982-0224-20140142
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| Formato: |
text/html
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| Fonte: |
Neotropical Ichthyology v.13 n.3 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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